pcmv vector Search Results


pcmv myc vectors  (TaKaRa)
96
TaKaRa pcmv myc vectors
Pcmv Myc Vectors, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cv003  (Sino Biological)
92
Sino Biological cv003
Cv003, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcmv ha expression vectors  (TaKaRa)
96
TaKaRa pcmv ha expression vectors
Pcmv Ha Expression Vectors, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dsred express  (TaKaRa)
95
TaKaRa dsred express
Dsred Express, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcmv  (TaKaRa)
94
TaKaRa pcmv
Pcmv, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcmv lacz plasmid  (TaKaRa)
93
TaKaRa pcmv lacz plasmid
Pcmv Lacz Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcmv tdtomato  (TaKaRa)
94
TaKaRa pcmv tdtomato
Construction of plasmid pVHSV expressing the complete antigenomic RNA of French strain VHSV 23-75 and insertion of a transcription cassette inserting <t>tdTomato</t> into the N-P intergenic region. Five overlapping cDNA fragments (numbered 1 to 5) covering the complete VHSV 23-75 French strain antigenome were generated by RT-PCR with primers described in Table ​Table11 and were assembled in pBluescript SK− following appropriate restriction enzyme digestion in the fragment order 5 to 1. In the final construct, named pVHSV, the leader end of the antigenome cDNA was flanked by the T7 promoter (T7), and the trailer end was fused to the hepatitis delta virus ribozyme (δ) followed by a terminator for T7 RNA polymerase (T7t). The complete antigenomic cDNA was designed and confirmed to be identical to the established VHSV 23-75 French strain sequence (accession number FN665788), except for four nucleotide substitutions that were introduced to create an SpeI restriction site in the M-G intergenic region. As described in Materials and Methods, an expression cassette encoding tdTomato was inserted in the N-P intergenic region using the unique PsiI enzyme restriction site, leading to the final construct pVHSV-Tomato. The additional gene end/gene start signal is boxed in gray.
Pcmv Tdtomato, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcmv tdtomato - by Bioz Stars, 2026-04
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pcmv dsred express2  (TaKaRa)
95
TaKaRa pcmv dsred express2
Construction of plasmid pVHSV expressing the complete antigenomic RNA of French strain VHSV 23-75 and insertion of a transcription cassette inserting <t>tdTomato</t> into the N-P intergenic region. Five overlapping cDNA fragments (numbered 1 to 5) covering the complete VHSV 23-75 French strain antigenome were generated by RT-PCR with primers described in Table ​Table11 and were assembled in pBluescript SK− following appropriate restriction enzyme digestion in the fragment order 5 to 1. In the final construct, named pVHSV, the leader end of the antigenome cDNA was flanked by the T7 promoter (T7), and the trailer end was fused to the hepatitis delta virus ribozyme (δ) followed by a terminator for T7 RNA polymerase (T7t). The complete antigenomic cDNA was designed and confirmed to be identical to the established VHSV 23-75 French strain sequence (accession number FN665788), except for four nucleotide substitutions that were introduced to create an SpeI restriction site in the M-G intergenic region. As described in Materials and Methods, an expression cassette encoding tdTomato was inserted in the N-P intergenic region using the unique PsiI enzyme restriction site, leading to the final construct pVHSV-Tomato. The additional gene end/gene start signal is boxed in gray.
Pcmv Dsred Express2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent protein e2 crimson  (TaKaRa)
93
TaKaRa fluorescent protein e2 crimson
Construction of plasmid pVHSV expressing the complete antigenomic RNA of French strain VHSV 23-75 and insertion of a transcription cassette inserting <t>tdTomato</t> into the N-P intergenic region. Five overlapping cDNA fragments (numbered 1 to 5) covering the complete VHSV 23-75 French strain antigenome were generated by RT-PCR with primers described in Table ​Table11 and were assembled in pBluescript SK− following appropriate restriction enzyme digestion in the fragment order 5 to 1. In the final construct, named pVHSV, the leader end of the antigenome cDNA was flanked by the T7 promoter (T7), and the trailer end was fused to the hepatitis delta virus ribozyme (δ) followed by a terminator for T7 RNA polymerase (T7t). The complete antigenomic cDNA was designed and confirmed to be identical to the established VHSV 23-75 French strain sequence (accession number FN665788), except for four nucleotide substitutions that were introduced to create an SpeI restriction site in the M-G intergenic region. As described in Materials and Methods, an expression cassette encoding tdTomato was inserted in the N-P intergenic region using the unique PsiI enzyme restriction site, leading to the final construct pVHSV-Tomato. The additional gene end/gene start signal is boxed in gray.
Fluorescent Protein E2 Crimson, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcmv hygro negative control vector flag tagged  (Sino Biological)
91
Sino Biological pcmv hygro negative control vector flag tagged
Construction of plasmid pVHSV expressing the complete antigenomic RNA of French strain VHSV 23-75 and insertion of a transcription cassette inserting <t>tdTomato</t> into the N-P intergenic region. Five overlapping cDNA fragments (numbered 1 to 5) covering the complete VHSV 23-75 French strain antigenome were generated by RT-PCR with primers described in Table ​Table11 and were assembled in pBluescript SK− following appropriate restriction enzyme digestion in the fragment order 5 to 1. In the final construct, named pVHSV, the leader end of the antigenome cDNA was flanked by the T7 promoter (T7), and the trailer end was fused to the hepatitis delta virus ribozyme (δ) followed by a terminator for T7 RNA polymerase (T7t). The complete antigenomic cDNA was designed and confirmed to be identical to the established VHSV 23-75 French strain sequence (accession number FN665788), except for four nucleotide substitutions that were introduced to create an SpeI restriction site in the M-G intergenic region. As described in Materials and Methods, an expression cassette encoding tdTomato was inserted in the N-P intergenic region using the unique PsiI enzyme restriction site, leading to the final construct pVHSV-Tomato. The additional gene end/gene start signal is boxed in gray.
Pcmv Hygro Negative Control Vector Flag Tagged, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flag vector  (Sino Biological)
93
Sino Biological flag vector
Construction of plasmid pVHSV expressing the complete antigenomic RNA of French strain VHSV 23-75 and insertion of a transcription cassette inserting <t>tdTomato</t> into the N-P intergenic region. Five overlapping cDNA fragments (numbered 1 to 5) covering the complete VHSV 23-75 French strain antigenome were generated by RT-PCR with primers described in Table ​Table11 and were assembled in pBluescript SK− following appropriate restriction enzyme digestion in the fragment order 5 to 1. In the final construct, named pVHSV, the leader end of the antigenome cDNA was flanked by the T7 promoter (T7), and the trailer end was fused to the hepatitis delta virus ribozyme (δ) followed by a terminator for T7 RNA polymerase (T7t). The complete antigenomic cDNA was designed and confirmed to be identical to the established VHSV 23-75 French strain sequence (accession number FN665788), except for four nucleotide substitutions that were introduced to create an SpeI restriction site in the M-G intergenic region. As described in Materials and Methods, an expression cassette encoding tdTomato was inserted in the N-P intergenic region using the unique PsiI enzyme restriction site, leading to the final construct pVHSV-Tomato. The additional gene end/gene start signal is boxed in gray.
Flag Vector, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flag vector - by Bioz Stars, 2026-04
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pcmv hygro  (Sino Biological)
90
Sino Biological pcmv hygro
Construction of plasmid pVHSV expressing the complete antigenomic RNA of French strain VHSV 23-75 and insertion of a transcription cassette inserting <t>tdTomato</t> into the N-P intergenic region. Five overlapping cDNA fragments (numbered 1 to 5) covering the complete VHSV 23-75 French strain antigenome were generated by RT-PCR with primers described in Table ​Table11 and were assembled in pBluescript SK− following appropriate restriction enzyme digestion in the fragment order 5 to 1. In the final construct, named pVHSV, the leader end of the antigenome cDNA was flanked by the T7 promoter (T7), and the trailer end was fused to the hepatitis delta virus ribozyme (δ) followed by a terminator for T7 RNA polymerase (T7t). The complete antigenomic cDNA was designed and confirmed to be identical to the established VHSV 23-75 French strain sequence (accession number FN665788), except for four nucleotide substitutions that were introduced to create an SpeI restriction site in the M-G intergenic region. As described in Materials and Methods, an expression cassette encoding tdTomato was inserted in the N-P intergenic region using the unique PsiI enzyme restriction site, leading to the final construct pVHSV-Tomato. The additional gene end/gene start signal is boxed in gray.
Pcmv Hygro, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Construction of plasmid pVHSV expressing the complete antigenomic RNA of French strain VHSV 23-75 and insertion of a transcription cassette inserting tdTomato into the N-P intergenic region. Five overlapping cDNA fragments (numbered 1 to 5) covering the complete VHSV 23-75 French strain antigenome were generated by RT-PCR with primers described in Table ​Table11 and were assembled in pBluescript SK− following appropriate restriction enzyme digestion in the fragment order 5 to 1. In the final construct, named pVHSV, the leader end of the antigenome cDNA was flanked by the T7 promoter (T7), and the trailer end was fused to the hepatitis delta virus ribozyme (δ) followed by a terminator for T7 RNA polymerase (T7t). The complete antigenomic cDNA was designed and confirmed to be identical to the established VHSV 23-75 French strain sequence (accession number FN665788), except for four nucleotide substitutions that were introduced to create an SpeI restriction site in the M-G intergenic region. As described in Materials and Methods, an expression cassette encoding tdTomato was inserted in the N-P intergenic region using the unique PsiI enzyme restriction site, leading to the final construct pVHSV-Tomato. The additional gene end/gene start signal is boxed in gray.

Journal: Journal of Virology

Article Title: Limited Interference at the Early Stage of Infection between Two Recombinant Novirhabdoviruses: Viral Hemorrhagic Septicemia Virus and Infectious Hematopoietic Necrosis Virus ▿ †

doi: 10.1128/JVI.00343-10

Figure Lengend Snippet: Construction of plasmid pVHSV expressing the complete antigenomic RNA of French strain VHSV 23-75 and insertion of a transcription cassette inserting tdTomato into the N-P intergenic region. Five overlapping cDNA fragments (numbered 1 to 5) covering the complete VHSV 23-75 French strain antigenome were generated by RT-PCR with primers described in Table ​Table11 and were assembled in pBluescript SK− following appropriate restriction enzyme digestion in the fragment order 5 to 1. In the final construct, named pVHSV, the leader end of the antigenome cDNA was flanked by the T7 promoter (T7), and the trailer end was fused to the hepatitis delta virus ribozyme (δ) followed by a terminator for T7 RNA polymerase (T7t). The complete antigenomic cDNA was designed and confirmed to be identical to the established VHSV 23-75 French strain sequence (accession number FN665788), except for four nucleotide substitutions that were introduced to create an SpeI restriction site in the M-G intergenic region. As described in Materials and Methods, an expression cassette encoding tdTomato was inserted in the N-P intergenic region using the unique PsiI enzyme restriction site, leading to the final construct pVHSV-Tomato. The additional gene end/gene start signal is boxed in gray.

Article Snippet: The EGFP gene then was replaced by the tdTomato gene from pCMV-tdTomato (Clontech) after digestion with SpeI and SnaBI enzymes. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1. caption a7 Construction of plasmid pVHSV expressing the complete antigenomic RNA of French strain VHSV 23-75 and insertion of a transcription cassette inserting tdTomato into the N-P intergenic region.

Techniques: Plasmid Preparation, Expressing, Generated, Reverse Transcription Polymerase Chain Reaction, Construct, Sequencing